Will Lemon or Lime Juice create a false positive result on a HPT?

If you happen to have a Facebook, you may be familiar with our TTC (Trying To Conceive) Group we have on there, basically tailored around the fascinating world of Pee Sticks! Well, we decided to conduct a little experiment with Lime and Lemon Juice to see if perhaps, (as rumor has it) either would create a false Line?!

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The TRUTH behind Evaporation Lines

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Image: www.wisegeek.com Graphics: Pee-On-A-Stick-Freak

 
Being an avid TTC’er (Trying To Conceive) while going through your journey, I’m sure you’ve come across the word evap – which is short for (Evaporation Line) ⇦ which you can get a good look at some actual ones here ★ especially if you’ve joined any support groups. Posting good HPT (Home Pregnancy Test) Pics is ultimately an art form nonetheless and I’ve even created a tutorial on (How to post GOOD HPT Pics) if you ever opt to get a second opinion on the results!

Posting pics can be great, the only downfall is the opinions and advice that ultimately goes along with it. Reading and interpreting results is not as easy as you would think.

First of all, (regardless of what test you’re choosing to take) you have to make sure that you read the instructions carefully beforehand. Not every test is the same, and most errors occur from not doing so. Some tests allow you to hold the absorbent tip in your Urine stream, while other tests require you to collect your Urine in a clean, sanitary cup beforehand. Also, reaction times can differ as well. Most Tests however, specify a certain amount of time to read the results and to disregard the test after that allotted timeframe. When taking a test, a Control Line should appear indicating that the test has been done correctly, and a Test Line will only appear if the Pregnancy hormone HCG (Human Chorionic Gonadotropin) is present inside of the Urine and has a high enough concentration for that specific test to pick up on as the hormone binds to the chemicals on the antibody strip – causing it to change colors. That would be considered a positive result and would mean that you are in fact pregnant! And Yes, even a very faint Line still means that there’s a presence of HCG in your system, even if it’s a very small amount. A faint Line should not be confused with an EVAP, (Evaporation Line) or an (Indent Line).

The problem is how so many people nowadays seem to misuse the word and throw it around without using it in it’s proper context, or by using the word without knowing it’s true meaning. Either way, I decided to once and for all clarify the term since there seems to be so much confusion behind it.

So, what is an Evap Line? And, how can you tell the difference? The true definition of an Evap sort of speaks for itself – it’s a Line that develops as the Urine evaporates off of the HPT. As the Urine dries, it can cause the antibody strip to appear slightly more visible, and in some cases can actually look like an indentation on the test itself. Most of the time they will appear shadowy, colorless or even grey. In some cases, they can however also develop a little bit of color. Although any Line that may look promising, (especially one with Blue or Pink) if it appears outside of the timeframe it should be considered invalid and disregarded. Since an Evap Line only occurs once the Urine has dried, it’s safe to say that any Line that appears within that allotted timeframe specified on the instructions would be considered a positive result no matter how faint the Line may be. If you’re strict on reading the test only within the timeframe per the manufacturer – the test should still be damp and no Evap Lines will have had time to produce. Most test instructions indicate that a test should not be read after the 3-10 minute time period.

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Image: www.pregnancyandbaby.com Graphics: Pee-On-A-Stick-Freak


 
Sometimes after we specify to others a pic we posted was snapped within the recommended timeframe we still come across those that say, “It looks like an Evap to me.” So, what if a faint Line occurs within the timeframe?

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Image: www.pregnancyandbaby.com Graphics: Pee-On-A-Stick-Freak


 
Again, more than likely that Evap Line your friend or co-worker thought they saw, really isn’t an Evap Line. Here’s a few examples of some actual Evaps I came across recently.

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Image: www.digitalmuze.net Graphics: Pee-On-A-Stick-Freak


 
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Image: www.justmommies.com Graphics: Pee-On-A-Stick-Freak


 
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Image: www.webanswers.com Graphics: Pee-On-A-Stick-Freaks


 
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Image: www.babybumpapp.com Graphics: Pee-On-A-Stick-Freak


 
If it’s a very faint Line, I always highly recommend to take another test in a couple days to track your Line Progression. Every 48 hours during early Pregnancy HCG will double as it should and over the course of a few days – you should start to see your test Lines get a little darker each time you pee on that stick! Of course with that being said, different Tests have different sensitivities that require different concentrations of the HCG in your Urine to produce a positive result. I highly recommend using the same brand and sensitivity to track your progress until you’re levels are high enough to be detected reliably on any test.

ALSO, something I felt I should discuss is the infamous, “Water Trick.” What is that you ask? Well, apparently somewhere along the wonderful internet we came across some topic or forum somewhere – where someone had mentioned that a great way to distinguish the Evap Line from a True Positive Result is by conducting the water trick. You simply put a few drops of water in the same place you would drop Urine and it’ll run across the test cleaning up the icky yellowish dried Urine on the test. This theory states that if it’s an Evap – than the line will wash away leaving the area pristine white again whereas if the line contains dye – then its a true BFP (Big Fat Positive) because any line with color even outside of the time limit MUST be positive according to some. Sorry to say that unfortunately that’s NOT always the case. EVAPS CAN HAVE COLOR and sometimes they DO NOT wash away! Here’s an example of just that, that actually came out of my personal collection recently! The line was very pink, and when I tried the water trick – the line stayed intact. So that myth has been busted! Sometimes it works, and sometimes it doesn’t. My best advice to you, is to toss the test outside once you’ve established it’s a negative result (within) the timeframe – and then proceed to run it over a few times! After that chunk it in a dumpster! Do whatever you have to do, but put the pee stick down and walk away. You can always retest later if you feel like you could still be pregnant!

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The next controversy we often encounter is the unwanted Indent Line, which is far too common and also can be misinterpreted as a positive result. More often than not, those usually appear outside of the timeframe – but not always! Occasionally they can appear within. But indent Lines unlike EVAP Lines, is simply the antibody strip. Indent Lines are usually completely colorless, or white when they appear within the timeframe. If you see an indent with color, or one with grey, than at that point it can then be considered an Evap if it appeared outside of the time limit. Here’s a few example those:

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Image: www.twoweekwait.com Graphics: Pee-On-A-Stick-Freak


 
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Image: www.twoweekwait.com Graphics: Pee-On-A-Stick-Freak


 
This however, is how you can distinguish the difference between an antibody strip or indent with the real deal below. The Line is Pink. It can be faint, but just Keep in mind the reagent in the dye binds to the chemicals on the antibody strip as the Urine passes over it when HCG is present. So a good rule of thumb, if it’s white or you need break the case open to see it, (which also makes the test invalid) than more than likely it’s not positive.

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Image: www.community.babycenter.com Graphics: Pee-On-A-Stick-Freak


 
One of the main reasons for seeing a False Line, (although rare) – is due to a dye run. That happens when the dye doesn’t run across the test smoothly and can get stuck on certain parts of the test. Whenever that happens, Sometimes it can cause a Line to appear where a true one would have had HCG been detected in the Urine. Although it looks very promising to the naked eye, it’s not considered to be a true positive. It doesn’t mean you’re not pregnant, it just simply means the result is unreliable since it was faulty  and if you suspect that you could still be pregnant, than you need to do yourself a favor and retest. Here’s a good example:

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Image: www.community.babycenter.com Graphics: Pee-On-A-Stick-Freak


 
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Then we have the famous, “Disappearing,” Line. Many of us get that confused with a true positive result. Although it, (again) seems promising, it’s not considered legitimate. This Sometimes happens when the dye is not fully finished running across the test. The dye can get stuck or may stall in the correct spot briefly, but then continues moving along. Per Most manufacturers – a true positive result will stay intact for at least 48 hours if not a whole lot longer! I definitely can vouch for that myself. I opted to save my Pregnancy Tests from both of my pregnancies (my son is now 4 and my daughter is 3) and every test Line to this day still remains as vivid as ever!

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Image: www.early-pregnancy-tests.com Graphics: Pee-On-A-Stick-Freak


 
And lastly, I feel the need to discuss the odd and unusual, (but not unheard of) test Lines that appear in the wrong spots on our lovely little pee sticks. Yes it happens. Yes it’s more than one Line. Does it mean it’s positive? No. Most of the time it occurs because of a dye run, and Sometimes the test is just faulty. The results should be considered (again) invalid and if you suspect your pregnant you should of course retest.

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Image: www.gaia.com Graphics: Pee-On-A-Stick-Freak


 
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Looks very odd, huh? And Yes it happens. But hopefully after reading this You’ll have a much better understanding of this and my goal more than anything else, was to hopefully reduce the phrase, “Evap,” from being so loosely thrown around as if any Line on any given test if faint enough or Maybe not even colorful enough in ones opinion – automatically means just that!

Moral of the story: Evap means that URINE actually EVAPORATED off of the test! And it also will ONLY appear outside of the time limit, so please use caution when using that term.

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How to Take a GOOD visible pic of your HPT

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Usually around the time of your missed period, you can take a HPT (Home Pregnancy Test) and get a reliable result. Some also opt to test early! Around 6-12dpo (Days Past Ovulation) implantation can occur, and a few days after that the HCG (Human Chorionic Gonadotropin) can metabolize enough in your Urine stream to then be detected on a HPT. If you’re actively TTC (Trying To Conceive) it can be a pretty exciting time, and you may want to get others opinions on whether or not they see a second Line, once you’ve tested. Many of us are a part of support groups and it’s fun to post pictures. The problem lies when the picture isn’t the best quality, and sometimes it can make it a bit difficult to interpret the results if you’re an outsider looking in. So I decided to make a little tutorial on how to take GOOD pics of your lovely pee sticks!

It’s always good for starters, to take a pic in good lighting! Most of us nowadays have good cell phones with camera’s – that even have a flash!

But – is it always best to use the flash? Simple answer, NO. If you’re using a midstream test that has a splash guard on it, (the clear plastic piece over the results window) the flash will more often than not reflect back and you won’t be able to see the results. Your best option is to turn the flash off. Sometimes though, that can cause another problem. Blurry pics! Here’s a pic that was taken with the flash on. Although it’s not Blurry, you still cannot see the results.

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Here’s a few examples of Blurry pics that will be very hard to interpret unless you have an unmistakable, very vivid, dark Test Line! Please try to refrain from posting these.

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There’s a very simple remedy for that issue! Maybe on your groups forums or posts you’ve noticed objects on or around their Tests and You’ve wondered why? Well – objects make the camera focus! Especially when you turn the flash off. It’s best to put a test in great lighting and an object next to it or even on it. Most with smart phones can even tap the screen to also help focus things. Here’s a few taken the right way! PLEASE (if you opt to post anything) only post pics that are good and visible otherwise it’ll defeat the purpose if you’re trying to get a second opinion.

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Now, I’m going to proceed to show you some other, “Picture Don’ts.” Here we have the ever so popular angled Tests. Unfortunately even in the best of lighting, it’s still very hard to see a second Line this way. Your best bet is to take the pic directly above the Tests!

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Love tweaking? What exactly is tweaking? Well, most of use different apps or graphics programs on our phones nowadays or even on our computers that can put different filters, enhance contrast, saturation, etc. On any pic of a HPT. The idea is to either pull some color on a line that is considered questionable, or to make a line a little more visible. Tweaking is great! The problem lies with over tweaking. And you can do that by using several different methods to try and enhance a test pic. Most don’t even realize though that when you over-tweak, you’re making it even harder to interpret. It can cause way too much pixilization with way too many colors, so if there was a chance to spot a line – there definitely won’t be afterwards. PLEASE, it’s better just to post the original and let the others do the tweaking!

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And of course the invert! If you are going to tweak, I would highly recommend to refrain from doing that. Inverts will make most ANY Line visible including (Indent Lines) and of course the dreadful (Evaporation Lines).

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If you look close enough at the inverted pic up above, you’ll see a Line. Looks promising, doesn’t it? Not so much. That’s an indent. So Yeah.

Even if you can’t test during the day when you’ll have the best lighting available – you can still make your pictures visible. Making sure the camera will focus correctly, and aiming directly right above the test is key!

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SUMMARY OF OUR WONDERFUL TUTORIAL HERE:

– Make sure you have good lighting! (Window seals are perfect for this type of thing)
– Make sure you turn your flash off
– Make sure you have an object on or near the test (to help the camera focus)
– Make sure you Take a pic directly up above the test, and as close to the results window as possible!

IN THE END (Regardless if it’s positive or Negative results) YOU WANT YOUR TEST PICS TO LOOK LIKE THIS!

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Nice, clean, bright and clearly visible! I hope this has helped you and we send Baby Dust to everyone in their TTC journey!

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The BEST Deals on Ovulation Tests and Pregnancy Tests

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If you’re actively TTC (Trying To Conceive) and especially for those of you who are (Charting) your fertility, (Basal Body Temperature, Cervical Mucus, Cervical Position and of course by also using Ovulation Predictor Kits in conjunction with those other primary fertility indicators) – You’re more than likely also wanting to test on or around the time of your expected period! And granted, if you’re an early tester it can get costly. HPT’s (Home Pregnancy Tests) come in all different forms such as Test Strips, Cassette Tests, Midstreams and even Digitals. Most of us are on a budget and I’ve made it my mission to find some of the absolute BEST DEALS that can make it way more affordable to Pee-On-That-Stick!

* PRODUCTS CAN BE PURCHASED OFF OF EBAY & AMAZON *

10miu HCG Test Strips:

Quantity: 30 Test Strips $6.99 + Free Shipping

Quantity: 20 Test Strips (with Free Collection Cups) $6.99 + Free Shipping

Quantity: 10 Test Strips $1.75 + Free Shipping

15miu HCG Test Strips:

Quantity: 50 Test Strips $6.99 + Free Shipping

25miu HCG Test Strips:

Quantity: 100 Test Strips $10.99 + Free Shipping

Quantity: 20 Test Strips $1.82 + Free Shipping

Quantity: 10 Test Strips $1.09 + $0.49 Shipping

Quantity: 10 Test Strips $0.99 + $0.59 Shipping

Quantity: 12 Test Strips $2.99 + Free Shipping

Quantity: 20 Test Strips $2.29 + Free Shipping

Quantity: 10/20/30 Test Strips $3.99 + Free Shipping

Quantity: 25 Test Strips $3.49 + Free Shipping

Quantity: 50 Test Strips $6.99 + Free Shipping

Midstream HCG Tests:

Quantity: 20 Midstream Tests (25miu) $11.80 + Free Shipping

Quantity: 5/10 Midstream Tests (25miu) $2.49 + Free Shipping

Cassette HCG Tests:

Quantity: 20 Cassette Tests (10miu) $7.99 + Free Shipping

Quantity: 50 Cassette Tests (25miu) $13.33 + $2.39 Shipping

Ovulation Test Strips:

Quantity: 100 MicroMall Test Strips (20miu) $15.90 + Free Shipping

Quantity: 40 Test Strips (25miu) $7.99 + Free Shipping

Quantity: 50 ClinicalGuard Test Strips (25miu) $9.99 + Free Shipping

Quantity: 10 Test Strips (25miu) $0.99 + $0.39 Shipping

Quantity: 10 LH Test Strips (15miu) $2.00 + Free Shipping

Combo Deals:

Quantity: 100 Blue Cross One Step LH / 20 HCG Test Strips (25miu) $20.99 + Free Shipping

Quantity: 50 LH / 20 HCG GetMore Test Strips (25miu) $8.99 + Free Shipping

Quantity: 60 LH / 30 HCG Test Strips (25miu) $13.69 + Free Shipping

Quantity: 50 LH / 20 HCG Strips $9.99 + Free Shipping

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Name Brand Pregnancy Tests:

Quantity: 3 First Response Early Results Midstream Tests (25miu but can detect 6.5miu) $12.95 + Free Shipping

Quantity: 8 Clearblue Digital Midstream Tests $21.50 + Free Shipping

Quantity: 9 E.P.T Midstream Tests (40miu) $25.00 + Free Shipping

Quantity: 6 E.P.T Midstream Tests (40miu) $7.50 + $2.50 Shipping

Quantity: 3 E.P.T Digital Tests (40miu) $7.49 + Free Shipping

Quantity: 6 Clearblue Plus Midstream Tests (25miu) $24.99 + Free Shipping

Quantity: 2 CVS Brand Digital Midstream Tests $9.99 + Free Shipping

Quantity: 3 CVS Brand Early Result Midstream Tests $4.99 + $1.93 Shipping

Quantity: 2 Answer Midstream Tests $7.77 + Free Shipping

DISCLAIMER: These may be limited time offers only! Prices and Shipping Fees are subject to change.

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How Urine Concentration effects OPK Results

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Many of us often opt to start using OPK’s (Ovulation Predictor Kits) to get a heads up on when Ovulation may occur. Our LH (Luteinizing Hormone) levels normally begin to rise just hours prior, and can be measured by using OPK’s. Normally the first true positive result – when the Test Line is equal to or Darker than the Control Line, indicates that Ovulation should be taking place anytime within 12-72 hours. But when using these, how will Urine concentration typically effect those results? It’s always recommended to use highly Concentrated (afternoon) Urine between 2-8pm at the same time everyday! Well, I decided to put it to the test. I dipped one OPK test Strip in Concentrated Urine, and then dipped a second one (same brand) around the same time minus an hour and a ½ in diluted Urine. See what happens next!

[youtube https://www.youtube.com/watch?v=fHRFh-A7Gt8&w=560&h=315]

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Equate OPK Customer Reviews!

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“Terrible!!!!! False Positives that are thin and only one side of the vertical line that sucks!!!! Don’t buy these or the Dollar Store Brands.”TTCWolfCub

“The walmart brand OV test are no good. my first time using it a couple test turned out invalid and also i got a positive off of one but when my blood results came back negative a couple days after. the walmart brands give false positives…i warn you…be careful…i have had a bad experience with it before.”Dori

“The local Wal-Mart didn’t have the ClearBlue Easy non-digitals that I usually get so I bought this product. Hey, all the Equate brands that I used were the same or better than the known brands. What could have gone wrong?

I followed the instructions in the box and four of the tests didn’t work! No matter if I peed in a cup or peed on the stick, nothing could register to make the test work. Because of this, I never was able to get a positive OPK. I will never use this brand again!” – jerrysliljess

“I have NEVER got a positive response on any. I’ve purchased two boxes and in both boxes half of them didn’t even work! I will not be wasting my money on these again!ADavid323

“It works okay, buy you’ll drive yourself crazy trying to guess whether the line is dark enough, to mean you’re ovulating. I abandoned these after a few months of confusion as to whether I was actually ovulating or about to ovulate, or what. I went back to the other brand that shows the smiley face when it’s time. The lack of guesswork was worth the extra money I saved on these. Actually I didn’t save anything with these because I had to use them for several months. I used the smiley face one once and got pregnant right away. Oh, by the way this is my second pregnancy, and both times started with this type of test to save money, but it was the smiley face that did it for me each time. Don’t waste your time, ladies!”LMC

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What Do I Record as Cycle Day 1? (The Difference Between SPOTTING and Your PERIOD)


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Image: www.healthtap.com Graphics: Pee-On-A-Stick-Freak

 

When you’re new to (Charting) it can be a bit confusing sometimes to know exactly WHEN or WHAT to Record on your Fertility Chart as CD (Cycle Day) 1. We used to assume that anytime you had any kind of bleeding, “Well that MUST be the start of our Period!” Yeah, ummmm, no matter how Light, Heavy or even when it happened!

I’m sure you’ve heard this from a friend or family member, “You must just be starting Early,” OR,  “It must be a LATE Period.” Better yet, no matter how Light it was, maybe they also told you – (or maybe you just assumed as so many of us do) that it was just an EXTREMELY LIGHT Period! Well, as time goes on more and more research is done to help expand our minds and give us some clarity!

There are two phases to our Cycle – The Follicular Phase (The time from the first day of our Menstrual Period until Ovulation takes place) and that’s controlled by a Hormone called, “Estrogen.” The next Phase of our cycle is known as The Luteal Phase (The time from Ovulation until either our Period starts or we achieve Pregnancy) and that’s controlled by a Hormone called, “Progesterone,” which is a heat inducing Hormone.

If you happen to take your BBT’s (Basal Body Temperatures) throughout your cycle, Estrogen keeps your temps low during the first Phase prior to Ovulation and Progesterone elevates and sustains our higher temps post Ovulation. Most of the time, (either one or two things will happen) – we will notice that our BBT’s have remained High past 13-16 DPO (Days Past Ovulation) and have possibly achieved Pregnancy! Or, our temps will drop back down which is a good indication that we can expect our Period sometime soon.

Sometimes though we can experience what is known as, “Mid-Cycle Spotting,” on or around Ovulation. That is not uncommon! Usually (if you’re also keeping track of your Cervical Fluid or Cervical Mucus) you may notice a bit of Fluid that resembles raw Egg Whites. If you happen to see a bit of Pink, Red, or even Brown tinged EWCM (Egg White Cervical Mucus) that is considered to be amongst the most fertile, and is caused Sometimes by a bit of spotting due to the Mature Egg breaking through the Egg Follicle.

At other times, (although it is considered RARE) you may notice a bit of spotting between 7-10 DPO and if you’ve achieved Pregnancy than that can also be known as, “Implantation Spotting,” which OFTEN gets mistaken for a Period! (Now, not ALL spotting is associated with Ovulation and Pregnancy) BUT – it can be considered as a clue, and can therefore give you a bit of insight. To actually peak into your Fertile Window – you may wanna consider taking a OPK (Ovulation Predictor Kit), also to confirm Early Pregnancy I would highly suggest taking a High Sensitivity HPT (Home Pregnancy Test!)

So, how do you differentiate between Spotting and actual Flo? Well, (as some licensed Doctors have put it) if you wipe and only see a bit of Pink, or a few drops of Blood – then that would be considered, “Spotting.”

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Images: www.wikihow.com Graphics: Pee-On-A-Stick-Freak


Any amount of RED Blood that requires a pad or a tampon would be considered, “Menses or actual Flo.”

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Image: www.wikihow.com Graphics: Pee-On-A-Stick-Freak


So essentially, if you need a pad or a tampon than you can officially record that as, “Cycle Day 1.”

Cycle Day 1 (again) is defined as your first day of actual Bright RED or Dark RED Flo that requires a Pad or a Tampon!

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Image: www.egeve.com Graphics: Pee-On-A-Stick-Freak

If you’re actively TTC (Trying To Conceive) and you have a higher Temperature above your coverline – (and you notice a bit of spotting) Don’t lose hope just yet! There may be a possibility that it’s simply Implantation Spotting.

If you’re new to Charting, (or tracking your Period) even if you’re NOT actively TTC – Charting is still a great way to pinpoint your Period each cycle and can also indicate whether or not you should possibly Test for Pregnancy.

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How long Do You Have to hold a HPT or OPK in urine?

So we you’re about to take a HPT (Home Pregnancy Test) or even a OPK (Ovulation Predictor Kit) – usually when you’re reading the instructions that come in the box, there’s a reccomended length of time they specify holding the Dip stick in the urine stream (or same difference if it’s a Midstream Test)! Honestly, I never really gave it much thought. Sometimes whenever I’m making these awesome, fun Videos for you guys, I can get a bit distracted, (say if one of my kiddos starts knocking on the door for example Ha Ha) and it may not be until I actually go back and watch the video that I catch something I hadn’t noticed during filming! When I first got the Equate Brand OPK I tested one with PM urine and being as it was a dreadful Blue Dye Test, I was a bit confused by the results! So maybe 15 mins later I dipped another one (in the SAME urine sample) and held it a few seconds longer. Guess what? The T (Test) line was darker than the C (Control) line! Whereas with the Test before, the 2 Lines were the SAME! Soooooooooo I concluded from that experience that it DOES matter how long you actually hold a Dip stick in the urine and (of course) how CONCENTRATED the urine sample is! I highly recommend holding the dip stick a minimal of 5-10 seconds! Anything less, may yield a much lighter result. Now keep in mind (whether it be a OPK or a HPT) a positive result is a positive result as long as the Tests are taken properly and read within the correct time frame. If you have a High enough concentration of HCG (Human Chorionic Gonadotropin) a HPT will yield a POSITIVE result, much like a OPK will if you have a high enough concentration of LH (Luteinizing Hormone) BUT – the Line opacity can differ! So, I will be conducting more Tests like this in the near future. But for now, I’m using the Blue Dye Tests and I hope you find this helpful! 

[youtube https://www.youtube.com/watch?v=urgvA2a0m-U&w=560&h=315]

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Positive OPK’s CD32 using Day 3 New Choice OPK

First Response OPK vs New Choice OPK!