Can the TEMPERATURE of URINE effect the result of a OPK?

As I was doing a Video Experiment yesterday, it occurred to me that there may be a possibility that the actual Temperature of Urine itself, could possibly effect the outcome of a OPK! As I was looking at the Tests (with the naked eye vs camera) they both yielded positive results BUT the T (Test) Line wasn’t as dark as it normally is on the Test used with CONCENTRATED Urine. I would have expected that with the Test used with DILUTED Urine. Of course going through my emails I actually came across one that had sparked my same interest and theory about THE TEMPERATURE OF URINE! Could that have had en effect on the result?

(Part 1)

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(Part 2)

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Can the CONCENTRATION of Urine effect the opacity of a Test Line on a OPK?!

I’ve had a few inquiries (Yes my fellow TTC’ers please keep the experiment ideas coming!) about whether or not the actual CONCENTRATION of urine, can effect the lightness or darkness of a Test Line? So, (seeing as how I’m not currently Pregnant) I thought, WHY NOT TEST WITH AN OPK? (Ovulation Predictor Kit) – For some odd reason during my last cycle I had an extremely higher level of LH (Luteinizing Hormone) and had tested positive the night before!

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