Can the CONCENTRATION of Urine effect the opacity of a Test Line on a OPK?!

I’ve had a few inquiries (Yes my fellow TTC’ers please keep the experiment ideas coming!) about whether or not the actual CONCENTRATION of urine, can effect the lightness or darkness of a Test Line? So, (seeing as how I’m not currently Pregnant) I thought, WHY NOT TEST WITH AN OPK? (Ovulation Predictor Kit) – For some odd reason during my last cycle I had an extremely higher level of LH (Luteinizing Hormone) and had tested positive the night before!

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Blue Dye vs Pink Dye OPK

How long Do You Have to hold a HPT or OPK in urine?

So we you’re about to take a HPT (Home Pregnancy Test) or even a OPK (Ovulation Predictor Kit) – usually when you’re reading the instructions that come in the box, there’s a reccomended length of time they specify holding the Dip stick in the urine stream (or same difference if it’s a Midstream Test)! Honestly, I never really gave it much thought. Sometimes whenever I’m making these awesome, fun Videos for you guys, I can get a bit distracted, (say if one of my kiddos starts knocking on the door for example Ha Ha) and it may not be until I actually go back and watch the video that I catch something I hadn’t noticed during filming! When I first got the Equate Brand OPK I tested one with PM urine and being as it was a dreadful Blue Dye Test, I was a bit confused by the results! So maybe 15 mins later I dipped another one (in the SAME urine sample) and held it a few seconds longer. Guess what? The T (Test) line was darker than the C (Control) line! Whereas with the Test before, the 2 Lines were the SAME! Soooooooooo I concluded from that experience that it DOES matter how long you actually hold a Dip stick in the urine and (of course) how CONCENTRATED the urine sample is! I highly recommend holding the dip stick a minimal of 5-10 seconds! Anything less, may yield a much lighter result. Now keep in mind (whether it be a OPK or a HPT) a positive result is a positive result as long as the Tests are taken properly and read within the correct time frame. If you have a High enough concentration of HCG (Human Chorionic Gonadotropin) a HPT will yield a POSITIVE result, much like a OPK will if you have a high enough concentration of LH (Luteinizing Hormone) BUT – the Line opacity can differ! So, I will be conducting more Tests like this in the near future. But for now, I’m using the Blue Dye Tests and I hope you find this helpful! 

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New Choice OPK vs Equate OPK

Positive OPK’s CD35 using a New Brand!

Going on CD35, and 7 days of BLAZING POSITIVES with (First Response – 100m/IU according to consumer studies) The Dollar Tree Brand (New Choice – 25m/IU) I decided to get yet a 3rd Brand! Of course my DH (Darling Husband) went to the wonderful world of Walmart and bought their (Equate – 100m/IU according to consumer studies) Ovulation Midstream Tests. But Yeah, they were the HORRIBLE BLUE DYE TESTS! Eeeeeeek! (Another story for another day!) So I thought I would give them a try……….

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Positive OPK’s CD34 using Day 5 New Choice OPK

Positive OPK’s CD33 using Day 4 New Choice OPK

Positive OPK’s CD32 using Day 3 New Choice OPK

Positive OPK’s CD31 using Day 2 New Choice OPK

First Response OPK vs New Choice OPK!